By Ronald Frank, Jens Schneider-Mergener (auth.), Joachim Koch, Michael Mahler (eds.)

Proteins interacting with diversified ligands - proteins, peptides or DNA - are the fundamental rules underlying many organic procedures, similar to antigen-antibody binding, sign transduction or receptor binding.
The means of oligopeptide synthesis on a cellulose membrane and the next binding assays enable the research of protein interactions. a selected benefit of those peptide arrays (SPOT - know-how) is the excessive variety of oligopeptide probes that may be demonstrated in parallel. specific protocols for peptide synthesis, and the research of protein-protein, protein-DNA interactions in addition to epitope mapping are awarded during this guide. it really is ultimate not just for uncomplicated examine laboratories but in addition for diagnostic and healing functions on account that many illnesses are concerning dysfunctions in protein popularity and binding.

Show description

Read or Download Peptide Arrays on Membrane Supports: Synthesis and Applications PDF

Similar nonfiction_9 books

Trace Gas Exchange in Forest Ecosystems

This quantity summarizes the present wisdom at the trade of hint gases among forests and the ambience with the restrict that solely carbon and nitrogen compounds are integrated. For this goal the amount brings jointly and interconnects wisdom from diverse disciplines of organic and atmospheric sciences.

Recoding: Expansion of Decoding Rules Enriches Gene Expression

The dynamic nature of interpreting the data in messenger RNA was once unanticipated on the time the genetic code used to be first deciphered. We now comprehend that either the which means of person codons and the framing of the readout method may be transformed by way of info in particular messenger RNAs. This publication describes this "Recoding" phenomenon, revealing the range of an extra layer of data in mRNAs that serves to counterpoint the expression of genes.

Observation of CP Violation in B± → DK± Decays

CP violation is a well-established phenomenon in particle physics, yet until eventually 2001 it used to be in basic terms saw in kaons. within the final decade, numerous matter-antimatter asymmetries were saw in impartial B mesons according to the expectancies of the normal version of the vulnerable interplay. Direct CP violation can also be anticipated within the decay premiums of charged B+ mesons as opposed to that of B- mesons, even though the best results are found in a decay that happens simply two times in 10 million decays.

The Vascular Endothelium I

This quantity deals a finished overview of contemporary advances in examine at the vascular endothelium. It offers a ancient heritage and a consultant for destiny instructions in learn. This publication includes 20 chapters via a various team of authors who've all made major contributions to the sphere of analysis within the vascular endothelium.

Extra info for Peptide Arrays on Membrane Supports: Synthesis and Applications

Example text

By following the stripping conditions (SDS, urea, acetic acid) described, these membranes can be reused 50 times and more (Frank and Owervin 1996). However, also with these membranes it is not advisable to use strongly acidic stripping conditions. The limited solvent compatibility of the cellulose membranes needs to be considered only if the peptides are to be modified during or after synthesis in solvents different from DMF, 1-methyl-2-pyrrolidone or water. Polar solvents are necessary to ensure a complete reaction while other common, less polar organic solvents like pyridine, THF or dichloromethane often lead to a low conversion.

In: Epton R (ed) Proc Int Symp on Innovation and Perspectives in Solid Phase Synthesis, Oxford, August 1993. Mayflower Worldwide Ltd, Birmingham,pp 509-512 Frank R, Giiler S (1990) Verfahren zur schnellen Synthese von tragergebundenen oder freien Peptiden oder Oligonucleotiden, damit hergestelltes 21 22 RoNALD FRANK, JENS ScHNEIDER-MERGENER Flachmaterial, dessen Verwendung sowie Vorrichtung zur Durchfiihrung des Verfahrens. 9 Frank R, Giiler S, Krause S, Lindenmaier W (1991) Facile and rapid spotsynthesis of large numbers of peptides on membrane sheets.

9. Wash off excess acetic anhydride with DMF 10. Start next cycle with step 2, or, after the last cycle, proceed with step 11. End ofthe synthesis 11. Perform a final Fmoc deprotection (steps 2 and 3). 12. Wash off excess piperidine with DMF and stain with BPB (step 4). 13. Acetylate the terminal amino groups with a mixture of 2 o/o (v/v) acetic anhydride in DMF (step 8). 14. Wash off excess acetic anhydride with DMF. 15. Wash membrane with ethanol and dry. 16. Cleave two times for 1 h with TFA/dichloromethane/water/ diisobutylsilane 50:50:2:3 (v/v).

Download PDF sample

Rated 4.83 of 5 – based on 35 votes