By A. D. Bangham, M. W. Hill, N. G. A. Miller (auth.), Edward D. Korn (eds.)

Examination of the tables of contents of journals - biochemical, molecular organic, ultrastructural, and physiological-provides convincing proof that membrane biology could be within the Nineteen Seventies what biochemical genetics was once within the Nineteen Sixties. And for strong cause. If genetics is the mechanism for major­ taining and transmitting the necessities of lifestyles, membranes are in lots of methods the essence of lifestyles. The minimum requirement for self sufficient life is the individualism supplied by means of the separation of "life" from the surroundings. The mobile exists through advantage of its floor membran~. One could outline the 1st dwelling organism as that degree of evolution the place macromolecular catalysts or self-reproducing polymers have been first segregated from the encompassing milieu by way of a membrane. no matter if that early membrane resembled current mobilephone membranes is inappropriate. What issues is membrane could have supplied a mechanism for conserving a neighborhood focus of molecules, facilitating chemical evolution and permitting it to adapt into biochemical evolution. That or but extra primitive membranes, equivalent to a hydrocarbon monolayer at an air-water interface, may also have supplied a floor that will facilitate the aggregation and particular orientation of molecules and catalyze their interactions. If primitive membranes have been even more than mere passive obstacles to unfastened diffusion, how even more is that this real of the membranes of up to date types of lifestyles. a massive revolution in organic concept has been the recogni­ tion that the mobile, and particularly the eukaryotic mobile, is a bewildering maze of membranes and membranous organelles.

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G. A. Miller permeant under study. If, for example, the marked permeant has a very low permeability such as 22Na + or 42 K +, then the method described by Bangham et al. (l965a) has some merit. 0 ml) of the liposome dispersion into lengths of wetted dialysis tubing tied off at their lower ends through a throat made from two concentric, snugly fitting lengths of polythene tubing which grips the dialysis tubing (Visking 21/32 inch). The inner polythene tube is then plugged. In batches of nine, these tubes are clipped to an all-polythene frame and placed into a I-liter-capacity polythene bottle containing approximately 500 ml of isotope-free isotonic solution.

There are two methods. ) and attach this via the amino group to whatever group is desired (Kornberg and McConnell, 1971). The other method is to use the reaction outlined by lost et al. (1971), which follows accepted procedures. Hubbell and McConnell (1971) give methods for labeling fatty acids and lecithin; their reaction is o [0] + ( R1 and R z are chosen to give the appropriate molecule and can be part of a ring, as with spin-labeled cholesterol. Resonance spectra were obtained by 32 A. D. Bangbam, M.

L965a) has some merit. 0 ml) of the liposome dispersion into lengths of wetted dialysis tubing tied off at their lower ends through a throat made from two concentric, snugly fitting lengths of polythene tubing which grips the dialysis tubing (Visking 21/32 inch). The inner polythene tube is then plugged. In batches of nine, these tubes are clipped to an all-polythene frame and placed into a I-liter-capacity polythene bottle containing approximately 500 ml of isotope-free isotonic solution. The bottle and contents are then rotated at 1 rpm.

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