By W. Neal Burnette, David W. Whiteley (auth.), M. Zouhair Atassi (eds.)
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Extra resources for Immunobiology of Proteins and Peptides V: Vaccines Mechanisms, Design, and Applications
Such~. , 1988). The pYA260. ~. coli lacZ gene has been cloned into pYA248 to yield the plasmid When pYA260 and pYA248 were introduced into various cloning hosts with a ßasd mutation in the chromosome ßasd strain X3llS and the ß~ ß~ and~. ~. coli typhimurium ßasd vaccine strain X4072, ß- galactosidase is synthesized constitutively and at high levels by cells with pYA260 (TAßLE S). ß~ ß~ It is evident from the data in TABLE S that the ßasd strain X4072 permits a higher level of ß-galactosidase expression than is achieved in other strains.
There are liIany otner vi rus pathogens for whi ch there are no, or only parti ally effecti ve vacci nes. Tllere isa great need, therefore, for vacci nes against these viruses. V Fi g. 1. Coval ent structures of surface pep ti des of the HA mol ecul e synthesized in our laboratory. In the synthetic peptides, the cysteine residues at positions 227, 281, and 305 are replaced with glycine. It is not suggested that the antigenic sites stretch the full length of these peptides but rather that the sites reside within the boundaries of these peptides.
Porin protein of Neisseria gonorrhoeae: Cloning and gene strueture. Proe. Natl. Acad. Sei. USA 84:8135-8139, 1987. 42. Gotsehlieh EC, Blake MS, Koomey JM, Seif M, Derman A. Cloning of the struetural genes of three H8 antigens and protein 111 of Neisseria gonorrhoeae. J. Exp. Med. 164:868881, 1986. 43. Gotsehlieh EC, Seiff M, Blake MS. The DNA sequenee of the struetural gene of gonoeoeeal protein 111 and the flanking region eontaining a repetitive sequenee. J. Exp. Med. 165:471-482, 1987. 31 STABLE RECOMBINANT AVIRULENT SALMONELLA VACCINE STRAINS Roy Curtiss 111, Sandra M.