By Natasha S. Barteneva, Ivan A. Vorobjev
This certain quantity for the 1st time explores concepts and protocols related to quantitative imaging circulation cytometry (IFC), which has revolutionized our skill to investigate cells, mobile clusters, and populations in a amazing type. starting with an creation to expertise, the booklet keeps with sections addressing protocols for stories at the cellphone nucleus, nucleic acids, and FISH suggestions utilizing an IFC tool, immune reaction research and drug screening, IFC protocols for apoptosis and phone dying research, in addition to morphological research and the id of infrequent cells. Written for the hugely profitable Methods in Molecular Biology sequence, chapters comprise introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, with no trouble reproducible laboratory protocols, and tips about troubleshooting and keeping off identified pitfalls.
Authoritative and sensible, Imaging circulate Cytometry: equipment and Protocols should be a serious resource for all laboratories looking to enforce IFC of their examine studies.
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Additional resources for Imaging Flow Cytometry: Methods and Protocols
2. Several mirrors for optical beam propagation alignment within the interferometer. 3. Compatible kinematic mounts, post holders, and posts for above optics. S. Lau et al. Fig. 5 (a) Schematic of the spectrally-encoded imaging module in time-stretch imaging. 5 Key Components for Time-Stretch Operation These are the components to perform wavelength-to-time mapping of the spectrally-encoded image data (either the ATOM or iTS signal) and thus enable ultrafast image capture: 1. ) 2. 5). 3. High-speed single-pixel photodetector (8 GHz, Picometrix).
At the time of this writing, the third-generation ImageStreamX Mark II is capable not only of object detection and image segmentation, but also real-time image registration, fluorescence compensation, and the calculation of a much broader and more sophisticated set of image features, despite the fact that it operates at nearly ten times the data rate of the original system. Thanks to Moore’s law, subsequent generations of instrumentation should be capable of the real-time determination of co-localization, spot counting, and other sophisticated image processing tasks at rates fast enough to enable cell sorting.
However, gaining access to image information compromises the measurement throughput. , charge-coupled devices (CCDs) or complementary metal oxide semiconductor (CMOS)) of optical microscope systems— higher imaging speed, thus throughput, is impeded by the loss of detectable light signal . This explains why the current state-ofthe-art imaging flow cytometers can only reach an imaging throughput of few thousands cells/s, compared with the throughput of ~100,000 cells/s of the classical non-imaging flow cytometers .