By J. D. Winefordner(eds.)

A multidisciplinary method of realizing the basics of mass spectrometry for bacterial analysis

From chemotaxonomy to characterization of certain proteins, id of Microorganisms by means of Mass Spectrometry offers an outline of either well-established and state of the art mass spectrometry suggestions for selecting microorganisms. a necessary device for microbiologists, healthiness execs, and analytical chemists, the textual content is designed to assist scientists opt for the simplest innovations to be used in biomedical, biochemical, pharmaceutical, and bioterror security applications.

in view that microbiological purposes of mass spectrometry require a simple realizing of either microbiology and analytical chemistry, the editors have integrated fabric from either disciplines in order that readers from both box will come to appreciate the mandatory rules of the opposite. that includes contributions from probably the most well-known specialists in either fields, this quantity presents particular examples of primary tools in addition to methods built within the final decade, including:
* Metastable atom bombardment pyrolysis mass spectrometry
* Matrix-assisted laser desorption/ionization mass spectrometry (MALDI)
* MALDI time-of-flight mass spectrometry (MALDI-TOF MS) of intact bacteria
* High-resolution Fourier remodel mass spectrometry (FTMS)
* Electrospray ionization (ESI) mass spectrometry

id of Microorganisms by means of Mass Spectrometry represents the main complete and up to date paintings at the subject presently on hand. it truly is liberally illustrated with figures and tables and covers each point of spectrometric identity of microorganisms, together with experimental systems, quite a few technique of pattern guidance, information research, and interpretation of advanced mass spectral data.Content:
Chapter 1 Cultural, Serological, and Genetic tools for identity of micro organism (pages 1–21): John B. Sutherland and Fatemeh Rafii
Chapter 2 Mass Spectrometry: id and Biodetection, classes discovered and destiny advancements (pages 23–37): Alvin Fox
Chapter three An advent to MALDI?TOF MS (pages 39–60): Rohana Liyanage and Jackson O. Lay
Chapter four the improvement of the Block II Chemical organic Mass Spectrometer (pages 61–89): Wayne H. Griest and Stephen A. Lammert
Chapter five strategy Reproducibility and Spectral Library meeting for swift Bacterial Characterization by means of Metastable Atom Bombardment Pyrolysis Mass Spectrometry (pages 91–124): Jon G. Wilkes, Gary Miertschin, Todd Eschler, Les Hosey, Fatemeh Rafii, Larry dashing, Dan A. Buzatu and Michel J. Bertrand
Chapter 6 MALDI?TOF Mass Spectrometry of Intact micro organism (pages 125–152): Jackson O. Lay and Rohana Liyanage
Chapter 7 improvement of Spectral Pattern?Matching methods to Matrix?Assisted Laser Desorption/Ionization Time?of?Flight Mass Spectrometry for Bacterial identity (pages 153–160): Kristin H. Jarman and Karen L. Wahl
Chapter eight reviews of Malaria via Mass Spectrometry (pages 161–180): Plamen A. Demirev
Chapter nine Bacterial pressure Differentiation through Mass Spectrometry (pages 181–201): Randy J. Arnold, Jonathan A. Karty and James P. Reilly
Chapter 10 Bacterial Protein Biomarker Discovery: A concentrated method of constructing Molecular?Based identity structures (pages 203–227): Tracie L. Williams, Steven R. Monday and Steven M. Musser
Chapter eleven High?Throughput Microbial Characterizations utilizing Electrospray Ionization Mass Spectrometry and Its function in useful Genomics (pages 229–256): Seetharaman Vaidyanathan and Royston Goodacre
Chapter 12 Bioinformatics for Flexibility, Reliability, and mix research of Intact Microorganisms (pages 257–277): Catherine Fenselau and Patrick Pribil
Chapter thirteen MALDI?FTMS of Whole?Cell micro organism (pages 279–300): Jeffrey J. Jones, Michael J. Stump and Charles L. Wilkins
Chapter 14 A evaluate of Antibody trap and Bacteriophage Amplification in reference to the Direct research of Whole?Cell micro organism by means of MALDI?TOF MS (pages 301–318): Kent J. Voorhees and Jon C. Rees
Chapter 15 Discrimination and id of Microorganisms by means of Pyrolysis Mass Spectrometry: From Burning pursuits to Cooling Embers—A old viewpoint (pages 319–343): Eadaoin Timmins and Royston Goodacre

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Extra resources for Identification of Microorganisms by Mass Spectrometry, Volume 169

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Karjalainen, E. , Hesso, A. , Jalonen, J. , Karjalainen, U. P. ). Amsterdam: Elsevier, 1998, Vol. 14, pp. 449–459. 12. ; Harrelson, D. Monitoring muramic acid in air (after alditol acetate derivatization) using a gas chromatography-ion trap tandem mass spectrometer. J. Microbiol. Meth. 1996, 27, 129–138. 13. ; Gray, B. M. Failure to detect muramic acid in normal rat tissues but detection in cerebrospinal fluid from patients with pneumococcal meningitis. Infect. Immun. 2000, 68, 4688–4698. REFERENCES 35 14.

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O. MALDI-TOF mass spectrometry of bacteria. Mass Spectrom. Rev. 2001, 20, 172–194. 37. Jones, J. ; Stump, M. ; Fleming, R. ; Lay, J. ; Wilkins, C. L. Investigation of MALDI-TOF and FTMS techniques for analysis of Escherichia coli whole cells. Anal. Chem. 2003, 75, 1340–1347. 38. ; Fenselau, C. Lectin-based affinity capture for MALDI-MS analysis of bacteria. Anal. Chem. 1999, 71, 1460–1463. 39. Madonna, A. ; Voorhees, K. J. Detection of Escherichia coli using immunomagnetic separation and bacteriophage amplification coupled with matrixassisted laser desorption/ionization time-of-flight mass spectrometry.

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