By John A. Sykes (auth.), Jørgen Fogh (eds.)
The research of cultured human tumor cells is a most evident process in experimental human melanoma learn. for plenty of concepts in virology, immunology, biochemistry, and biophysics, for instance, quite a lot of cells could be required and such amounts tend to be supplied simply whilst the cultures turn into demonstrated phone strains; whilst this occurs, thorough characterization additionally turns into attainable. the improvement of mobile strains, for this reason, is of leading significance. contemporary significant advances in study with animal mobile platforms see m to be a prologue for current and destiny efforts directed towards paintings with human tumor cells in tradition. Conceivably, the main major ends up in melanoma examine might enhance from paintings with such cells, and so the time appeared correct to outline the current country of our wisdom. this can be the 1st e-book devoted completely to the topic: human tumor cells in vitro. even supposing so me of the elemental elements within the cultivation of human tumor ceIls, and the level to which they signify human melanoma in vivo are nonetheless uncertain, I requested a few of the top investigators during this quarter of analysis to assemble and assessment earlier and current contributions, and to supply their techniques at the inquiries to which solutions will not be but on hand. some of the chapters are thinking about suggestions of cultivation. Cultures from a few different types of tumors have grown weIl; in lots of instances they've got given upward push to validated cellphone lines.
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Additional info for Human Tumor Cells in Vitro
Of interest is that the higher the concentration of amino acids used, the less need of the cells for serum supplements. Ling and coworkers found that using a medium with 54 mM of amino acids per liter was ne ar optimum for the serum-free cultures they established. Lg/ml cortisol, was chosen as the base medium to develop a "cancer cell" medium for the following reasons: I. The medium utilizes the free base amino acids, especially arginine, as the buffer system instead of sodium bicarbonate. The cells can grow in free gas exchange with the atmosphere, and this negates the necessity for special incubators.
1966), but it would be useless once we added collagenase. We therefore are testing the pectin compounds, because these glycoproteins appear to have a similar function in the plant kingdom as collagen in the animal kingdom. Agarose is used in very low concentrations for a possible structural lattice; microbiologists have long known that low concentrations of agar facilitate the growth of fastidious organisms in fluid media. Catalase is used to neutralize peroxidases after the findings of Lieberman and Ove (1959).
Grey, C. , 1964, Density gradient centrifugation studies of the Bittner virus, Texas Rep. Biol. Med. 22:609-627. Sykes, J. , Nolan, J. , 1970a, Some properties of a new epithelial celliine of human origin, J. Nati. Cancer Inst. 45:107-122. Sykes, J. , and Anson, J. , 1970b, Separation of tumor cells from fibroblasts with use of discontinuous density gradients, J. Natl. Cancer Inst. 44:855-864. , and Thon, I. , 1959, Isolation of "biologically intact" mask cells, Exp. Cell Res. 18:512-520. Walder, A.