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2006). The most potent chemicals need only be present at trace concentrations to induce biological effects, and often 18 Valentina F. Domingues, Virgínia C. Fernandes, Luísa Correia-Sá et al. are at or below analytical detection limits. , 2006). Instrumental methods are reliable, but they have several potential drawbacks, such as expensive instrumentation, complex derivatization, extensive clean-up, and purification, and they also require a very high level technical expertise for operation. High cost and low throughput limit the use of these techniques (Swart and Pool, 2007).

It is designed, in fact, for retaining both hydrophilic and hydrophobic compounds with high capacity by means of both van der Waals and H-donor-H acceptor interactions. , 2012). A comparison of the various types of SPE sorbents, protocols, and devices has been made by Lopez de Alda and Barcelo (Lopezde Alda and Barcelo, 2001). In this paper various procedures for the determination of several estrogens (estriol, E2, ethynyl estradiol, estrone, and diethylstilbestrol) and progestogens (progesterone, norethindrone, and levonorgestrel) in the environmental matrices, including water and river sediment, are described (Lopezde Alda and Barcelo, 2001).

Induction of phytoestrogen production in Medicago sativa leaves by irrigation with sewage water. Environmental and Experimental Botany, 1995 35, 3: 363-369. Shore, L. S. and M. Shemesh. Naturally produced steroid hormones and their release into the environment. Pure and Applied Chemistry, 2003 75, 11-12: 1859-1871. Silva, C. , M. Otero and V. Esteves. Processes for the elimination of estrogenic steroid hormones from water: A review. Environmental Pollution, 2012 165: 38-58. , S. J. Hyllner and C.

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