Bioprocessing: a thrilling new engineering self-discipline. It combines the improvement and optimization of biotechnological methods with potent ideas to recuperate and purify the specified items. safeguard in addition to rate play an immense position here.
This quantity covers the immensely differentiated spectrum of recommendations and operations of bioprocessing, provided via the main efficient specialists within the box. an summary of upstream and downstream processing is given, fermentation and mobile tradition tactics and the layout of microbial fermenters are offered. A last crew of chapters is devoted to problems with approach validation, size, and regulation.
issues integrated are: commercial phone Cultures/ Pharmaceutical Proteins/ Bioreactors/ Media and Air Sterilization/ Oxygen move/ Scale Implications/ Fermentation information research/ mobile and particles elimination/ Protein Purification/ Electrokinetic Separations/ ultimate restoration Steps/ technique Validation
Chapter 1 Fermentation: an summary (pages 7–22): Barry C. Buckland and Malcolm D. Lilly
Chapter 2 business Animal cellphone tradition (pages 23–38): Brian D. Kelley, Tzyy?Wen Chiou, Morris Rosenberg and Daniel I. C. Wang
Chapter three assessment of Downstream Processing (pages 39–55): Ron Spears
Chapter four Proteins and Peptides as medicines: assets and techniques of Purification (pages 57–73): Stuart E. Builder, Robert L. Garnick, James C. Hodgdon and John R. Ogez
Chapter five Bioreactors: Description and Modelling (pages 77–104): Jens Nielsen and John Villadsen
Chapter 6 cellphone tradition Bioreactors (pages 105–126): Athanassios Sambanis and Wei?Shou Hu
Chapter 7 Media for Microbial Fermentations (pages 127–139): Randolph L. Greasham
Chapter eight Media for phone tradition (pages 141–156): R. A. Wove
Chapter nine Media and Air Sterilization (pages 157–184): Gokaraju ok. Raju and Charles L. Cooney
Chapter 10 Oxygen move and combining: Scale?Up Implications (pages 185–217): Matthias Reuss
Chapter eleven Aeration in mobile tradition Bioreactors (pages 219–281): John G. Aunins and Hans?Jurgen Henzler
Chapter 12 suggestions for Fermentation with Recombinant Organisms (pages 283–294): Tadayuki Imanaka
Chapter thirteen Anaerobic Fermentations (pages 295–318): Larry E. Erickson, Daniel Y. C. Fung and Pravate Tuitemwong
Chapter 14 Fermentation tracking and keep an eye on (pages 319–354): Thomas Chattaway, Gary A. Montague and A. Julian Morris
Chapter 15 Fermentation facts research for analysis and regulate (pages 355–400): Gregory Stephanopoulos, Konstantin Konstantinov, Urs Saner and Toshiomi Yoshida
Chapter sixteen layout of Aseptic, Aerated Fermentors (pages 401–426): Marvin Charles and Jack Wilson
Chapter 17 Biotransformations and Enzyme Reactors (pages 427–466): Andreas S. Bommarius
Chapter 18 phone and mobile particles elimination: Centrif Ugation and Crossflow Filtration (pages 469–503): Rajiv V. Datar and Carl?Gustaf Rosen
Chapter 19 mobilephone Disruption and Isolation of Non?Secreted items (pages 505–526): Horst Schutte and Maria?Regina Kula
Chapter 20 In vitro Protein Refolding (pages 527–555): Jeffrey L. Cleland and Daniel I. C. Wang
Chapter 21 Liquid?Liquid Extraction (Small Molecules) (pages 557–592): Karl Schugerl
Chapter 22 Protein Purification through Liquid?Liquid Extraction (pages 593–616): Brian D. Kelley and T. Alan Hatton
Chapter 23 Protein Separation and Purification (pages 617–642): Jan?Christer Janson and Lars Ryden
Chapter 24 Affinity Separations (pages 643–677): Srikanth Sundaram and Martin L. Yarmush
Chapter 25 Electrokinetic Separations (pages 679–693): Alan J. Grodzinsky and Martin L. Yarmush
Chapter 26 ultimate restoration Steps: Lyophilization, Spray?Drying (pages 695–714): Christian F. Golker
Chapter 27 Analytical Protein Chemistry (pages 717–737): Srikanth Sundaram, David M. Yarmush and Martin L. Yarmush
Chapter 28 Biotechnology Facility layout and technique Validation (pages 739–767): Michael G. Beatrice
Chapter 29 therapy of organic Waste (pages 769–787): Daniel F. Liberman
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Extra info for Biotechnology: Bioprocessing, Volume 3, Second Edition
1978), Determination of mixing times in large-scale fer546-550. mentors using radioactive isotopes, Proc. 1st PACKER,H . , THOMAS,C. R. (1990), Morphological measurements on filamentous microorEur. Congr. , Part 11, 80-82. ganisms by fully automated image analysis, BioLILLY,M. D. (1983), Problems in process scale-up, technol. Bioeng. 35, 870-881. in: Bioactive Microbial Products 2 (WINSTANLILLY,M. LEY,D. , NISBET,L . ), pp. 79-89, Lon- PACKER,H . , THOMAS,C. R. (1992), Estimation of cell don: Academic Press.
Pp. 25-41, New York: Academic Press. , OGAWA,M. (1992), “Lights Out” production of cephamycins in automated fermentation facilities, in: Harnessing Biotechnology for the 21st Century (LADISCH,M. ), pp. 223-227, Washington, DC: American Chemical Society. EINSELE,A. , July, 13-14. , PETRE, J. FEHRENBACH, 0. (1992), On-line biomass monitoring by capaciAcknowledgements tance measurement, J. Biotechnol. 23, 303-3 14. The authors wish to thank Drs. MAIGETTER, FISH, N. , LILLY,M. D. (1984), The interactions MASUREKARand MONAGHANfor numerous between fermentation and protein recovery, Bio/ helpful editorial comments.
The sequence shown in Fig. 8 implies that new equipment will be required. This will depend on whether or not there is existing fermentation capacity and recovery equipment available in the production plant. Although in recent years some companies have installed new fermentors, many new products are introduced without the need for new 20 I Fermentation: An Overview plants. Whenever possible, companies rely on improvements in titers of existing products to release sufficient fermentation capacity in time for introduction of the next new product.