By Richard J. Geider, Bruce A. Osborne (auth.)

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3 g·dm- 3 sodium carbonate solutions. Working solutions should be stored in acid-cleaned Teflon bottles. 7. All pipette tips used to dispense 14C solutions should be rinsed three times in 2molm- 3 redistilled nitric acid, and six times with Milli-Q water prior to use. 25 cm- 3 ) of 14C stock solutions are used to further reduce potential contamination. 8. Plastic gloves and lab coats should be worn at all times when injecting samples. *Strong acids which modify the surface characteristics of polycarbonate bottles should be avoided.

A major advantage of measuring open-water TCO z fluxes rather than Oz fluxes is that TCO z exchanges slowly with the atmosphere in most natural waters and thus atmospheric corrections are minimal or unnecessary. An additional advantage in studies on coral reefs is that calcification and organic carbon fluxes can be obtained (Kinsey, 1985). However, the TCO z flux includes contributions from chemosynthetic CO z fixation and fermentation in addition to oxygenic photosynthesis and respiration. A disadvantage of measuring TCO z fluxes is that the analytical precision is Net Gas Exchange 29 generally less than can be obtained for O 2 measurements.

32) Iron-mediated, light-dependent O 2 consumption has been demonstrated for several organic compounds containing amino, carboxyl, and hydroxyl groups (Stumm and Morgan, 1970; Miles and Brezonik, 1981), whereas some other compounds, such as tannic acid, promote O 2 consumption in both light and darkness in the pH range 2-11 (Miles and Brezonik, 1981). , 1984). Lanne et a1. 2-/Lm pore membrane filters and implicated singlet oxygen production as a factor in the photooxidation. Higher rates of O 2 consumption were observed in quartz bottles and blood transfusion bags than in glass bottles, presumably because of greater penetration of ultraviolet radiation in the former.

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